The Unforgotten Pre-service Teachers [Participants]: Did the Pandemic Affect Learning While on Practicum? What Uncompleted Pre-service Teachers' Mentoring Experiences [Data] Can Tell Us

In education, practical, experiential, or experimental research is usually based on a number of participants from whom we collect data. We contact them and explain their role in the study and ask them to kindly devote their time to us. Our final dataset (data "useful for our research”) is a portion of our data corpus (all data collected). The story we finally print is the one from the dataset purposefully selected from the data corpus. The myriad of reasons given for not including some of our participants' data in the dataset include the following: participants' lack of enthusiasm, punctuality, timely delivery of audio recorded data, or data quality. The pandemic outbreak impacted my participants' lives, workplaces, and studies. Their practicum experience was impacted too, so some of them could not complete their own teaching rounds. As a consequence, they could not provide enough data to analyse in order to answer my research questions. They tried, but it was not possible for some of them. However, all the data collected, my data corpus, is valuable to me as a teacher educator and as a mentor teacher. This is a twofold story: my participants' troubles and commitment during their practicum, and my own troubles collecting data while not neglecting what my unselected participants did for me. The pandemic's devastating effects in teacher education programmes in Chile is affecting pre-service teachers' practicum experience, their relationship with their students and their relationship with their mentor teachers, both university-based tutors and school-based mentors. Knowing the needs and experiences of pre-service teachers who did not get a completed experience in their allocated university-partner schools may help us to better navigate the practicum experience in the present times. © The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd. 2022.

A Quality Improvement Project Impacts Resident Satisfaction with the Depart Process

Purpose of Study The current depart process resulted in slow work-flow and patient safety and equity concerns. The QI project aimed to improve resident satisfaction with the hospital discharge process. Methods Used The QI project was designed using the Model for Improvement. Starting April 2020, Plan-Do-Study-Act cycles included: hiring clinical team coordinators;creating standard depart instructions for diabetic ketoacidosis, pyelonephritis, seizures, croup and dehydration;uploading instructions to Powerchart;and clinician reminders to use instructions with families. Measures examined monthly, included resident satisfaction and patient readmissions. Summary of Results Resident satisfaction improved from 4.8 (February 2020) to 7.8 (August 2020) for the overall discharge process;from 5.3 to 7.9 for family education on all key points;from 6.0 to 7.7 for smooth transition of care;from 3.0 to 7.4 for no language barriers;and from 3.0 to 6.7 for no unnecessary delays, on a scale of 0/strongly disagree/ terrible to 10/strongly agree/excellent. Readmissions also trended downward. Conclusions During this QI project to address the depart process, resident satisfaction improved and readmissions declined. These results are encouraging, but should be interpreted in the context of decreased patient census due to COVID-19 and non-respiratory season, which may have decreased workload and increased education time and interpreter access. Next steps include PDSAs related to health literacy and Spanish translation.

Graphical-mathematical model to estimate CO2 concentration level as an indicator of SARS-CoV-2 transmission

The first documented cases of Coronavirus disease of 2019 (COVID-19) in Wuhan city (Hubei province, China) were caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), studies mention viral transmission is more common in indoor and poorly ventilated environments compared to outdoor environments or environments with abundant airflow. In this context, this research aimed to estimate, through a mathematical model by a Box-Behnken design, the time and occupancy required in a physical space to reach CO2 levels that exceed the risk level established as a safer condition of 700 ppm for SARS-CoV-2 transmission. According to the proposed mathematical model, it is possible to predict safe conditions. With this, it was found that natural ventilation is the best option to reduce CO2 concentration, considering the occupancy/m3 and time, allowing a constant airflow;the use of air conditioners to control the temperature in rooms without natural ventilation is suggested;notwithstanding, these types of equipment are not designed to reduce CO2 concentration. Thus, their use in rooms with open windows and doors leads to a shorter equipment lifetime, for this reason, its operation in special conditions, such as in rooms without natural ventilation should be considered

SARS-COV-2 VIRAL LOAD IN GOIÂNIA WASTEWATER: WASTEWATER-BASED EPIDEMIOLOGY FOR A COVID-19 EARLY WARNING SYSTEM. COVID-19

Thus, several tools have been used in the last two years to contain the spread of SARS-CoV-2. In this perspective, Wastewater-Based Epidemiology (WBE) provides valuable data by including symptomatic, pre-symptomatic, and asymptomatic individuals and anticipating clinical cases. For 37 weeks, the SARS-CoV-2 viral load was monitored in sanitary sewage samples from Goiânia-Brazil. Through pre-concentration, extraction, and purification of viral RNA, followed by RT-qPCR, it was detected in 83.78% (31/37) of the weeks the presence of fragments of the genetic material SARS-CoV-2 with viral loads ranging from 105 to 108 genome copies L-1. The viral load in the wastewater samples was related to the clinical data of the municipality, demonstrating its ability to promote an Early Warning System (EWS). In early 2022, it was possible to predict the increase in clinical cases 3 weeks in advance and issue an alert note to the health authorities. Prevalence estimates ranged from 0.003 to 0.5% and showed good agreement with observed values. Thus, monitoring effluents become another essential tool that can help combat the pandemic's advance, especially in countries with scarce resources. © 2022 Sociedade Brasileira de Quimica. All rights reserved.

Super effective antimicrobial silver-sputtered coatings on poly(lactic acid) against bacteria and omicron SARS-CoV-2

Poly(lactic acid) (PLA) is a biopolymer with properties potentially suitable for fabricating packaging, medical devices, and healthcare products in a more friendly environmental way because this polymer presents biodegradability, compostability, low carbon footprint, and recyclability. However, PLA does not present intrinsic antimicrobial properties. Antimicrobial materials are highly desirable for manufacturing smart packaging and personal protective equipment to secure food and health professionals against pathogenic microorganisms. In this work, we evaluated the antimicrobial performance of (Ag)-coated PLA against Escherichia coli, Bacillus subtilis, and Omicron severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). PLA was rapidly coated with metallic Ag by pulsed direct current magnetron sputtering (pDCMS) for 5, 10, and 20 s. Atomic force microscopy indicates that the Ag coating grows predominantly on the PLA surface via a bulk diffusion mechanism. According to bactericidal and quantitative reverse transcription polymerase chain reaction assays, Ag-coated PLA was capable of inhibiting bacterial biofilm formation and disrupting the genetic material of the Omicron SARS-CoV-2. X-ray high-resolution photoelectron and nuclear magnetic resonance results suggest no polymer chain scission in the PLA bulk due to plasma thermal stress effects during Ag sputtering. © 2023 Elsevier Ltd

Economies of community in local agriculture: farmers in New London, Connecticut, respond to the COVID-19 pandemic

The COVID-19 pandemic highlighted the weaknesses of the U.S. national food system, with grocery store shelves emptied in March and April 2020 and COVID outbreaks reported throughout the summer of 2020 at meat processing plants across the country. Fleetingly, Americans turned to local farms to ensure they could access food safely in a time of uncertainty. This paper examines the economies of community that formed around local farms and how direct engagements between consumers and producers in the face of the pandemic deepened these economic structures that often put community well-being above profits. Within a capitalist system that prioritizes efficient mass production, economies of community illustrate that solidarity can improve local food system resilience. Based on qualitative and quantitative research carried out in the summer of 2020 in New London County in southeastern Connecticut, this research draws on ethnographic interviews with small-scale farmers who developed innovative ways to feed some of their community's most vulnerable members. Community economies show that we should not only depend on standardized large-scale farms and giant retail distribution;the American food system needs to continue to cultivate small-scale local production in order to improve resilience and food access. At present, the sustainability of producing and distributing food occurs at the farmer's expense. The government needs to support local food producers so they can continue to play an integral part in community well-being.

Impact of relative humidity on COVID-19 transmission

Introduction: The new pathogen SARS-CoV-2 presents a rapid spread, and is the cause of the Coronavirus disease 2019 (COVID-19). Given that this virus is recent, the increase in cases of the COVID-19 disease has been a great threat, to which has been added the lack of conclusive information about the disease, its transmission, and its relationship with the different environmental variables.

The Great Retention: How to Keep the Best Employees Working in Your Facility

Husbandry staff retention and development are vital to the successful operation of an animal research facility. High levels of employee turnover can negatively affect the morale of the team and service continuity for researchers, as well as increase the workload for the remaining employees. Critical turning points that put additional stress on an animal facility, such as the COVID 19 pandemic, can magnify the situation and further increase the sense of burnout among the remaining staff. To increase the likelihood of employee retention and encourage husbandry staff to pursue internal promotions, our leadership team has focused on 4 key points to meet these goals: provide opportunities for continuing education, emphasis on effective communication with the staff, ensure adequate recognition of employee contributions to the team, and create a psychologically safe culture where coaching and mentoring is used to minimize the need for formal disciplinary action. As a result of implementing these strategies, there has been a substantial increase in internal promotions and a decrease in resignations, having a significant operational impact.

Strategy for the reactivation of tourism in Mexico;Evidence of the use of saliva pools for the detection of COVID-19 in a tourist resort

The COVID-19 pandemic has caused a decrease in tourism activity. This has caused huge losses to the tourism industry, so different approaches have been devised for economic reactivation. This article aims to provide a novel strategy for mass molecular monitoring of clinically healthy individuals, and also including potentially asymptomatic carriers of SARS-CoV-2, who traveled to a tourist resort The monitoring consisted of collecting individual saliva samples (n=120) and conforming groups of 10 samples, thus setting 12 individual pools, which were analyzed by qRT-PCR. Obtained data showed that all the samples analyzed were negative, thus confirming that no individuals were infected with SARS-CoV-2 before and during the travel. The proposed molecular protocol allowed the analysis of massive saliva samples and detect individual infected persons (negative or positive). protocol for evaluate massive event, thus promoting the economic activation of the tourism industry.

Monitoramento Da Carga Viral De Sars-Cov-2 Em ?Guas Residuais Na Cidade De Goi?Nia: Epidemiologia Baseada Em Esgoto E Um Sistema De Alerta Precoce Para Covid-19

SARS-COV-2 VIRAL LOAD IN GOIaNIA WASTEWATER: WASTEWATER-BASED EPIDEMIOLOGY FOR A COVID-19 EARLY WARNING SYSTEM. COVID-19 is currently the most critical disease in the world. Thus, several tools have been used in the last two years to contain the spread of SARS-CoV-2. In this perspective, Wastewater-Based Epidemiology (WBE) provides valuable data by including symptomatic, pre-symptomatic, and asymptomatic individuals and anticipating clinical cases. For 37 weeks, the SARS-CoV-2 viral load was monitored in sanitary sewage samples from Goiania-Brazil. Through pre-concentration, extraction, and purification of viral RNA, followed by RT-qPCR, it was detected in 83.78% (31/37) of the weeks the presence of fragments of the genetic material SARS-CoV-2 with viral loads ranging from 105 to 108 genome copies L-1. The viral load in the wastewater samples was related to the clinical data of the municipality, demonstrating its ability to promote an Early Warning System (EWS). In early 2022, it was possible to predict the increase in clinical cases 3 weeks in advance and issue an alert note to the health authorities. Prevalence estimates ranged from 0.003 to 0.5% and showed good agreement with observed values. Thus, monitoring effluents become another essential tool that can help combat the pandemic's advance, especially in countries with scarce resources.

NEW ZOONOTIC CORONAVIRUS PANDEMIC: THE APPLICATION OF WASTEWATER-BASED EPIDEMIOLOGY (WBE) FROM THE PERSPECTIVE OF ONE HEALTH

The new zoonotic coronavirus (SARS-CoV-2) was detected in China in late 2019 and, since then, it has spread rapidly, culminating in the pandemic. Studies show that symptomatic or asymptomatic individuals infected with SARS-CoV-2 can eliminate the virus through feces and urine, making it possible to detect viral RNA in excreta and, consequently, in wastewater. One Health is the inseparable union of animal, human and environmental health and within this concept, it is possible to use an environmental sample (wastewater) to monitor the circulation of the new coronavirus in a population, whether in companies, schools, neighborhoods, and municipalities. In this context, the present study aimed to demonstrate the successful use of WBE through a contingency plan model adopted in a company (Itaipu Hydroelectric Power Plant -IHPP) located in the city of Foz do Iguaçu, Brazil. Thus, statistical analysis and modeling were applied, using the results of weekly monitoring, between June/2020 and September/2021, of 10 representative points of each sector of the IHPP. During 65 monitored weeks, 500 mL of samples were sent conditioned on ice to the Federal University of ABC for detection and quantification of RNA fragments of the new coronavirus by reverse transcription technique followed by quantitative polymerase chain reaction (RT-qPCR). An occurrence variation of 7.7 and 2.6 log10 copies of RNA.L-1 was observed, and it is possible to correlate with the tendency of an increase in the number of cases in the IHPP and in the city of Foz. The results demonstrated the viability of the WBE approach in monitoring the new coronavirus, being a useful tool for decision-makers with the epidemiological surveillance and the health department. This approach may also be useful in detecting outbreaks caused by other waterborne pathogens not only in the human population but also in the companion animal population.

Association between telemedicine use and diabetes risk factor assessment and control in a primary care network.

PURPOSE: Our study examined whether telemedicine use in primary care is associated with risk factor assessment and control for patients with diabetes mellitus. METHODS: This was a retrospective, 1:1 propensity score matched cohort study conducted in a primary care network between February 2020 and December 2020. Participants included patients with diabetes mellitus, ages 18 to 75. Exposure of interest was any telemedicine visit. We determined whether hemoglobin A1c (HbA1c), blood pressure (BP) and low-density lipoprotein cholesterol (LDL-C) were assessed for each patient. For each risk factor, we also determined whether the risk factor was controlled when they were assessed (i.e., last HbA1c < 8.0%, BP < 130/80 mmHg, LDL-C < 100 mg/dL). RESULTS: After 1:1 propensity score matching, we identified 1,824 patients with diabetes during the study period. Telemedicine use was associated with a lower proportion of patients with all three risk factors assessed (162/912 [18%], versus 408/912 [45%], p < 0.001). However, when individual risk factors were assessed, telemedicine use did not impact risk factor control. When compared with patients with in-person visit only, the odds ratio (OR) for HbA1c < 8% was 1.04 (95% CI 0.74 to 1.46, p = 0.23) for patients with any telemedicine visit. Similarly, the OR for BP < 130/80 mmHg was 1.08 (95% CI 0.85-1.36 p = 0.53), and the OR for LDL-C < 100 mg/dL was 1.14 (95% CI 0.76-1.72, p = 0.52). CONCLUSIONS: Telemedicine use was associated with gaps in risk factor assessment for patients with diabetes during the COVID-19 pandemic, but had limited impact on whether risk factors were controlled.

Cotton Fabrics Decorated with Antimicrobial Ag-Coated TiO2 Nanoparticles Are Unable to Fully and Rapidly Eradicate SARS-CoV-2

The successful development of multifunctional cotton fabrics with antimicrobial and antiviral activities is essential to prevent the proliferation of microorganisms and transmission of coronavirus virions today, especially with the emergence of new variants of SARS-CoV-2. In this work, we developed antimicrobial cotton fabrics with Ag/TiO2 nanoparticles synthesized via sonochemistry. Here, we show that more than 50% of infectious SARS-CoV-2 remain active after prolonged direct contact self-disinfecting materials capable of inhibiting the proliferation of Escherichia coli and Staphylococcus aureus. The findings bring several epidemiologic worries about using silver and TiO2 as self-disinfecting nanostructured agents to prevent coronavirus transmission.

[Cardiac tamponade and myocarditis in a patient with acute SARS-CoV-2 infection]

We describe a patient with COVID-19, with pericardial effusion, cardiac tamponade and severe myocardial depression. A 51-year-old woman, previously healthy, with mild COVID-19 presented with three episodes of syncope. She was admitted to the emergency room. An electrocardiogram showed sinus rhythm, diffuse superior concavity ST 2 mv;a CT scan showed pericardial effusion, without lung pathological findings. Due to shock, dyspnoea and encephalopathy, the patient was admitted to intensive care, where she received vasopressor support and mechanical ventilation. A bedside ultrasound showed pericardial effusion and tamponade;drainage was performed;transoesophageal ultrasound showed moderate deterioration of biventricular systolic function;global longitudinal strain -14.2%, estimated Fey 43%;global circumferential strain -10.1%. Seven days after admission, CT scan revealed bilateral infiltrates and pericardial thickening with post-contrast enhancement and mild pericardial effusion. On day 12 post admission, inotropic support was discontinued;patient on mechanical ventilation weaning and haemodynamically stable.

Ex vivo treatment with fucoidan of mononuclear cells from SARS-CoV-2 infected patients.

COVID-19 is a worldwide health emergency, therapy for this disease is based on antiviral drugs and immunomodulators, however, there is no treatment to effectively reduce the COVID-19 mortality rate. Fucoidan is a polysaccharide obtained from marine brown algae, with anti-inflammatory, antiviral, and immune-enhancing properties, thus, fucoidan may be used as an alternative treatment (complementary to prescribed medical therapy) for the recovery of COVID-19.  This work aimed to determine the effects of ex-vivo treatment with fucoidan on cytotoxicity, apoptosis, necrosis, and senescence, besides functional parameters of calcium flux and mitochondrial membrane potential (ΔΨm) on human peripheral blood mononuclear cells isolated from SARS-CoV-2 infected, recovered and healthy subjects. Data suggest that fucoidan does not exert cytotoxicity or senescence, however, it induces the increment of intracellular calcium flux. Additionally, fucoidan promotes recovery of ΔΨm in PBMCs from COVID-19 recovered females. Data suggest that fucoidan could ameliorate the immune response in COVID-19 patients.

Covid-19 infection in patients with hematological diseases in Brazil: Clinical characteristics and prognostic factors

Background: The COVID-19 pandemic had a high burden in Brazil. To date, data on mortality and prognostic factors of COVID-19 infection in Brazilian patients with hematological disorders are scarce. Aims: To describe the characteristics and outcomes of patients with hematological disorders admitted to the hematological COVID care unit of a reference center in Brazil;to analyze the impact of prognostic factors on in-hospital mortality. Methods: This prospective, single-center study,included 118 patients who have been admitted to the hematological COVID care unit of the Hospital das Clínicas da Faculdade de Medicina da USP, S.o Paulo, Brazil, from March to September 2020.All patients had >18 years,an underlying hematological disease and a moderate or severe COVID- 19 infection.For analyses, patients were grouped in:(1)benign or no oncological treatment(n=43),(2) intensive chemotherapy,including induction protocols for acute leukemia and stem cell transplantation conditioning(n=44) or(3) intermediate chemotherapy,including lymphoma regimens,myeloma triple treatment or continuous treatment( n=31).The primary outcome was in-hospital mortality;secondary outcome was overall survival after admission in the COVID-19 unit.Univariate analysis(UVA) used odds ratio(OR) for baseline characteristics and ROC curve analysis for laboratory tests collected at admission.Multivariate analyses(MVA) were adjusted by age and hematological disease status group.The median follow-up and survival time after COVID-19-unit admission were estimated by Kaplan- Meier method.All statistical tests were two-sided;p-values<0.05 were considered significant. Results: Median age was 58(19-90) years and 55% of patients were male. Most patients(83%)had hematological malignancies,- mainly non-Hodgkin lymphoma(29%) and multiple myeloma(19%). The most frequent benign disease was sickle cell disease(5%).12 patients had undergone hematopoietic stem cell transplantation (HSCT),4 allogeneic and 8 autologous.70% had at least one comorbidity, mostly arterial hypertension and diabetes mellitus. Thromboembolic events occurred in 9%. Median hospital stay in the COVID-19 unit was 12(1-63) days;54% needed intensive care and 41% mechanical ventilation.In-hospital mortality rate was 41%[95%CI 32-50];most deaths occurred in patients with malignancies. Median follow-up was 73(95%CI 61-81) and 54(95%CI 39-66) days after admission and discharge from the COVID-19 unit, respectively.UVA showed a risk of death increased by 25% every 10 years old.The risk of in-hospital death was 3-fold and 5-fold higher in groups 2 and 3 compared with group 1.MVA showed higher risk of death in patients from group 2(OR=11.1,95% CI 2.9- 54.8) or group 3(OR=9.7,95%CI 2.4-47.5]),who had lactate dehydrogenase( LDH)>440 U/L(OR=16.8,95%CI 4.9-71.8),C-reactive protein(CRP)>100 mg/L(OR=4.1,95%CI 1.4-13.6) or platelet count<150x10e9/L(OR=3.7,95%CI 1.3-11.7), regardless of age(OR=1.2,95%CI 1.0-1.5).79% of in-hospital deaths were from COVID-19;others were mainly due to hematological disease.The overall median survival time after admission was 92 days(95% CI 34-NA) and the 75-day survival probability was 51%(95%CI 41-60).25% of patients had hospital readmission,mostly due to other infections. Summary/Conclusion: In line with other reports,patients with hematological diseases are at higher risk of mortality from COVID-19 infection, particularly in low and middle income countries.In our cohort, prognostic factors were status of disease,platelets count,LDH and CRP. These findings might help risk stratification and prioritization of vaccines in this setting.

Kidney disease and the coronavirus pandemic 2019 covid-19. An clinical perspective

Since the last months of 2019, SARS-CoV-2 was identified as a highly contagious virus, March 11, 2020 WHO classified SARS-CoV-2 as a pandemic. Since April 21, 2020 Mexican Government declared Phase 3 of his Contingency plan to fight SARS-CoV-2 pandemic. SARS-CoV-2 is mainly a respiratory virus, but there are reports of involvement in organs distant from the lungs, including the kidneys. SARS-CoV-2 kidney involvement is sign of worse prognosis. Is important to know the characteristics of this injury, the way to diagnose it, its treatment and how to prevent it. Here we review some theories about kidney injury related to SARS-CoV-2 infection and its histopathological correlation. We also evaluate the relationship between SARS-CoV-2 and Angiotensin Converting Enzyme 2 receptors, the kidney injury related to the use of the novels therapies against SARS-CoV-2, renal replacement therapies prescription in SARS-CoV-2 y in kidney transplant patients. Since the lasts months SARS-CoV-2 has invaded and modified our knowledge and daily routine. Healthcare staff from the different specialties, including Nephrologist must keep in constant learning and training to fight against this pandemic with the better weapons.